Response to comment on 'A conserved strategy for inducing appendage regeneration in moon jellyfish, Drosophila , and mice'.

Previously we reported evidence that a regenerative response in the appendages of moon jellyfish, fruit flies, and mice can be promoted by nutrient modulation (Abrams et al., 2021). Sustar and Tuthill subsequently reported that they had not been able to reproduce the induced regenerative response in flies (Sustar and Tuthill, 2023). Here we discuss that differences in the amputation method, treatment concentrations, age of the animals, and stress management explain why they did not observe a regenerative response in flies. Typically, 30-50% of treated flies showed response in our assay.
Competing Interests: YL, AS, IL, ZC, MR, JD, DG No competing interests declared, FT, MA, MH, LG Has applied for a patent related to the findings reported in this manuscript (US Patent Application No. 17/355,727)
(© 2023, Li et al.)