Back to Search Start Over

P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation.

Authors :
Trias I
Saco A
Marimon L
López Del Campo R
Manzotti C
Ordi O
Del Pino M
Pérez FM
Vega N
Alós S
Martínez A
Rodriguez-Carunchio L
Reig O
Jares P
Teixido C
Ajami T
Corral-Molina JM
Algaba F
Ribal MJ
Ribera-Cortada I
Rakislova N
Source :
Cancers [Cancers (Basel)] 2023 May 11; Vol. 15 (10). Date of Electronic Publication: 2023 May 11.
Publication Year :
2023

Abstract

p53 immunohistochemistry (IHC) has been proposed as a surrogate for TP53 mutations in penile squamous cell carcinomas (PSCC). We aimed to evaluate the performance of a pattern-based evaluation of p53 IHC in PSCC. Human papilloma virus (HPV) DNA testing, p16 and p53 IHC, and whole exome sequencing were performed in a series of 40 PSCC. p53 IHC was evaluated following a pattern-based framework and conventional p53 IHC evaluation. Out of 40 PSCC, 12 (30.0%) were HPV-associated, and 28 (70.0%) were HPV-independent. The agreement between the p53 IHC pattern-based evaluation and TP53 mutational status was almost perfect (k = 0.85). The sensitivity and accuracy of the pattern-based framework for identifying TP53 mutations were 95.5% and 92.5%, respectively, which were higher than the values of conventional p53 IHC interpretation (54.5% and 70.0%, respectively), whereas the specificity was the same (88.9%). In conclusions, the pattern-based framework improves the accuracy of detecting TP53 mutations in PSCC compared to the classical p53 IHC evaluation.

Details

Language :
English
ISSN :
2072-6694
Volume :
15
Issue :
10
Database :
MEDLINE
Journal :
Cancers
Publication Type :
Academic Journal
Accession number :
37345055
Full Text :
https://doi.org/10.3390/cancers15102719