A protocol for visualization of murine in situ neurovascular interfaces.

Mapping cranial vasculature and adjacent neurovascular interfaces in their entirety will enhance our understanding of central nervous system function in any physiologic state. We present a workflow to visualize in situ murine vasculature and surrounding cranial structures using terminal polymer casting of vessels, iterative sample processing and image acquisition, and automated image registration and processing. While this method does not obtain dynamic imaging due to mouse sacrifice, these studies can be performed before sacrifice and processed with other acquired images. For complete details on the use and execution of this protocol, please refer to Rosenblum et al. ¹ .
Competing Interests: Declaration of interests J.S.R., Vikram Chandrashekhar, and Vibhu Chandrashekhar are affiliated with Neurosimplicity, LLC, which is a medical device and technology company focusing on medical image processing. The content of this manuscript does not necessarily reflect the views, policies, or opinions of the U.S. Department of Health and Human Services. The mention of commercial products, their source, or their use in connection with material reported herein is not to be construed as an actual or implied endorsement of such products by the United States Government. Neurosimplicity, LLC has patent applications directed to the technology described in this manuscript.
(Published by Elsevier Inc.)