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Sensitive LC-MS/MS method for the temporal profiling of bile acids, fatty acids and branched-chain alpha-keto acids in maternal plasma during pregnancy and cord blood plasma at delivery.

Authors :
Ng DZW
Lee SXY
Ooi DSQ
Ta LDH
Yap GC
Tay CJX
Huang CH
Tham EH
Loo EXL
Shek LPC
Goh A
Bever HPSV
Teoh OH
Lee YS
Yap F
Tan KH
Chong YS
Chan SY
Eriksson JG
Godfrey KM
Lee BW
Chan ECY
Source :
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2023 Jul 01; Vol. 547, pp. 117449. Date of Electronic Publication: 2023 Jun 17.
Publication Year :
2023

Abstract

Background and Aims: There are significant changes to the maternal inflammatory profile across pregnancy. Recent studies suggest that perturbations in maternal gut microbial and dietary-derived plasma metabolites over the course of pregnancy mediate inflammation through a complex interplay of immunomodulatory effects. Despite this body of evidence, there is currently no analytical method that is suitable for the simultaneous profiling of these metabolites within human plasma.<br />Materials and Methods: We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the high-throughput analysis of these metabolites in human plasma without derivatization. Plasma samples were processed using liquid-liquid extraction method with varying proportions of methyl tert-butyl ether, methanol, and water in a 3:10:2.5 ratio to reduce matrix effects.<br />Results: LC-MS/MS detection was sufficiently sensitive to quantify these gut microbial and dietary-derived metabolites at physiological concentrations and linear calibration curves with r <superscript>2</superscript>  > 0.99 were obtained. Recovery was consistent across concentration levels. Stability experiments confirmed that up to 160 samples could be analyzed within a single batch. The method was validated and applied to analyse maternal plasma during the first and third trimester and cord blood plasma of 5 mothers.<br />Conclusion: This study validated a straightforward and sensitive LC-MS/MS method for the simultaneous quantitation of gut microbial and dietary-derived metabolites in human plasma within 9 minutes without prior sample derivatization.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-3492
Volume :
547
Database :
MEDLINE
Journal :
Clinica chimica acta; international journal of clinical chemistry
Publication Type :
Academic Journal
Accession number :
37331549
Full Text :
https://doi.org/10.1016/j.cca.2023.117449