Use of Azospirillum baldaniorum cells in quercetin detection.

Authors :: Kanevskiy MV
Kosheleva IS
Menukhov VO
Zhdanova ES
Borisova SV
Burygin GL
Konnova SA
Bunin VD
Guliy OI
Source :: ADMET & DMPK [ADMET DMPK] 2023 Mar 21; Vol. 11 (2), pp. 277-291. Date of Electronic Publication: 2023 Mar 21 (Print Publication: 2023).
Publication Year :: 2023
Abstract: The possibility of detection and determination of flavonoids by using microbial cells was shown for the first time using the quercetin - Azospirillum baldaniorum Sp245 model system. The activity of the flavonoids quercetin, rutin and naringenin toward A. baldaniorum Sp245 was evaluated. It was found that when the quercetin concentration ranged from 50 to 100 μM, the number of bacterial cells decreased. Rutin and naringenin did not affect bacterial numbers. Quercetin at 100 μM increased bacterial impedance by 60 %. Under the effect of quercetin, the magnitude of the electro-optical signal from cells decreased by 75 %, as compared with the no-quercetin control. Our data show the possibility of developing sensor-based systems for the detection and determination of flavonoids.<br />Competing Interests: Conflict of interest: The authors declare no conflict of interest.<br /> (Copyright © 2023 by the authors.)