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High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler.

Authors :
Sych T
Schlegel J
Barriga HMG
Ojansivu M
Hanke L
Weber F
Beklem Bostancioglu R
Ezzat K
Stangl H
Plochberger B
Laurencikiene J
El Andaloussi S
Fürth D
Stevens MM
Sezgin E
Source :
Nature biotechnology [Nat Biotechnol] 2024 Apr; Vol. 42 (4), pp. 587-590. Date of Electronic Publication: 2023 Jun 12.
Publication Year :
2024

Abstract

We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5-200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.<br /> (© 2023. The Author(s).)

Details

Language :
English
ISSN :
1546-1696
Volume :
42
Issue :
4
Database :
MEDLINE
Journal :
Nature biotechnology
Publication Type :
Academic Journal
Accession number :
37308687
Full Text :
https://doi.org/10.1038/s41587-023-01825-5