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[Mitogenic activity of the antigens isolated from different Staphylococcus aureus strains in relation to mouse and guinea pig splenocytes].
- Source :
-
Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 1986 May (5), pp. 67-73. - Publication Year :
- 1986
-
Abstract
- The mitogenic effect of corpuscular antigens with respect to the splenocytes of animals was found to depend on the strain of Staphylococcus aureus. The maximum synthesis of DNA in the cells was induced by corpuscular antigen Smith and the minimum synthesis, by Wood-46. The synthesis of DNA was activated in both B- and T-splenocytes in response to corpuscular antigens Wood-46, Cowan-1 and Smith, as well as to the cell wall and protein A. Peptidoglycan produced a mitogenic effect only in B-lymphocytes, and teichoic acid showed no mitogenic activity in mouse splenocytes. The mitogenic effect of staphylococcal antigens on splenocytes depended on the dose of the antigen and the time of cultivation. After 48-hour cultivation the incorporation of 3H-thymidine into the DNA of mouse cells was 5 times higher than into the DNA of guinea-pig cells. The optimum mitogenic dose in thymectomized BALB/c mice with respect to splenocytes was higher than in normal BALB/c mice practically by one order.
- Subjects :
- Animals
Cell Wall immunology
DNA biosynthesis
Dose-Response Relationship, Drug
Dose-Response Relationship, Immunologic
Guinea Pigs
Lymphocytes drug effects
Lymphocytes metabolism
Mice
Mice, Inbred BALB C
Mice, Nude
Mitogens isolation & purification
Peptidoglycan pharmacology
Spleen metabolism
Staphylococcal Protein A pharmacology
Stimulation, Chemical
Teichoic Acids pharmacology
Antigens, Bacterial isolation & purification
Mitogens pharmacology
Spleen drug effects
Staphylococcus aureus immunology
Subjects
Details
- Language :
- Russian
- ISSN :
- 0372-9311
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhurnal mikrobiologii, epidemiologii i immunobiologii
- Publication Type :
- Academic Journal
- Accession number :
- 3727880