Three new furanones from endophytic fungus Hypoxylon vinosopulvinatum DYR-1-7 from Cinnamomum cassia with their antifungal activity.

Hypoxylon vinosopulvinatum DYR-1-7 is a endophytic fungus isolated from the Cinnamomum cassia Presl and has an inhibitory effect on Lasiodiplodia pseudotheobromae . Three new furanones, hypoxylonone A-C ( 1 - 3 ), as well as three known compounds ( 4 - 6 ), were isolated from an EtOAc extract of H. vinosopulvinatum DYR-1-7. The structures were determined by spectroscopic data analysis using UV, IR, 1D-, 2D-NMR and HR-ESI-MS. The absolute configurations of 1 - 3 were elucidated by electronic circular dichroism (ECD) analyses. In the antifungal bioassay, Hypoxylonone B and C exhibited strong inhibitory effects on L. pseudotheobromae with IC ₅₀ value at the concentration of 1.01 and 2.40 μg/mL, respectively. Compound 6 showed medium antifungal activity with IC ₅₀ value at the concentration of 10.67 μg/mL on Fusarium oxysporum . Compounds 3 and 4 displayed medium antifungul effects on Candida albicans .