Back to Search
Start Over
Subcellular location of phosphoenolpyruvate carboxykinase in hepatocytes from fed and starved rats.
- Source :
-
The Journal of nutrition [J Nutr] 1986 Jun; Vol. 116 (6), pp. 1101-8. - Publication Year :
- 1986
-
Abstract
- To evaluate published indications that about 25% of the gluconeogenic enzyme, phosphoenolpyruvate carboxykinase (PEPCK), is located in mitochondria of adult rat liver, cell fractionations were conducted with hepatocytes isolated from rats that were fed ad libitum or starved for 2 days. Hepatocytes were exposed to digitonin for 10 s, and the released materials were separated from residual cell structures by centrifugation through a layer of brominated hydrocarbon. In addition to PEPCK, activities of 9 other enzymes were measured in the untreated cells and with good recovery in the two fractions obtained with digitonin treatment. By comparison with the release of marker enzymes for the cytosol and mitochondria, the subcellular distribution of PEPCK was determined. With cells from either fed or 2-day-starved rats, this enzyme was released exactly like lactate dehydrogenase and within 2-3% of phosphoglycerate kinase and pyruvate kinase. These results indicate that, even after induction by starvation, at least 97% of PEPCK activity is located in the cytosol of rat liver.
- Subjects :
- Animals
Aspartate Aminotransferases metabolism
Cell Fractionation
Cytosol enzymology
Digitonin pharmacology
L-Lactate Dehydrogenase metabolism
Liver drug effects
Male
Mitochondria, Liver enzymology
Phosphoglycerate Kinase metabolism
Pyruvate Kinase metabolism
Rats
Rats, Inbred Strains
Liver ultrastructure
Phosphoenolpyruvate Carboxykinase (GTP) metabolism
Starvation enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3166
- Volume :
- 116
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 3723205
- Full Text :
- https://doi.org/10.1093/jn/116.6.1101