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Analyzing the Genome-Wide Distribution of Histone Marks by CUT&Tag in Drosophila Embryos.

Authors :
Zenk F
Cardamone F
Ibarra Morales DA
Atinbayeva N
Zhan Y
Iovino N
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2655, pp. 1-17.
Publication Year :
2023

Abstract

CUT&Tag is a method to map the genome-wide distribution of histone modifications and some chromatin-associated proteins. CUT&Tag relies on antibody-targeted chromatin tagmentation and can easily be scaled up or automatized. This protocol provides clear experimental guidelines and helpful considerations when planning and executing CUT&Tag experiments.<br /> (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Details

Language :
English
ISSN :
1940-6029
Volume :
2655
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
37212984
Full Text :
https://doi.org/10.1007/978-1-0716-3143-0_1