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Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters.

Authors :
Stempels FC
Jiang M
Warner HM
Moser ML
Janssens MH
Maassen S
Nelen IH
de Boer R
Jiemy WF
Knight D
Selley J
O'Cualain R
Baranov MV
Burgers TCQ
Sansevrino R
Milovanovic D
Heeringa P
Jones MC
Vlijm R
Ter Beest M
van den Bogaart G
Source :
The Journal of cell biology [J Cell Biol] 2023 Jul 03; Vol. 222 (7). Date of Electronic Publication: 2023 May 18.
Publication Year :
2023

Abstract

Endosomal Sorting Complex Required for Transport (ESCRT) proteins can be transiently recruited to the plasma membrane for membrane repair and formation of extracellular vesicles. Here, we discovered micrometer-sized worm-shaped ESCRT structures that stably persist for multiple hours at the plasma membrane of macrophages, dendritic cells, and fibroblasts. These structures surround clusters of integrins and known cargoes of extracellular vesicles. The ESCRT structures are tightly connected to the cellular support and are left behind by the cells together with surrounding patches of membrane. The phospholipid composition is altered at the position of the ESCRT structures, and the actin cytoskeleton is locally degraded, which are hallmarks of membrane damage and extracellular vesicle formation. Disruption of actin polymerization increased the formation of the ESCRT structures and cell adhesion. The ESCRT structures were also present at plasma membrane contact sites with membrane-disrupting silica crystals. We propose that the ESCRT proteins are recruited to adhesion-induced membrane tears to induce extracellular shedding of the damaged membrane.<br /> (© 2023 Stempels et al.)

Details

Language :
English
ISSN :
1540-8140
Volume :
222
Issue :
7
Database :
MEDLINE
Journal :
The Journal of cell biology
Publication Type :
Academic Journal
Accession number :
37200023
Full Text :
https://doi.org/10.1083/jcb.202205130