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Engineered dual affinity protein fragments to bind collagen and capture growth factors.
- Source :
-
Materials today. Bio [Mater Today Bio] 2023 Apr 22; Vol. 20, pp. 100641. Date of Electronic Publication: 2023 Apr 22 (Print Publication: 2023). - Publication Year :
- 2023
-
Abstract
- Collagen type I lacks affinity for growth factors (GFs) and yet it is clinically used to deliver bone morphogenic protein 2 (BMP-2), a potent osteogenic growth factor. To mitigate this lack of affinity, supra-physiological concentrations of BMP-2 are loaded in collagen sponges leading to uncontrolled BMP-2 leakage out of the material. This has led to important adverse side effects such as carcinogenesis. Here, we design recombinant dual affinity protein fragments, produced in E. Coli , which contain two regions, one that spontaneously binds to collagen and a second one that binds BMP-2. By adding the fragment to collagen sponges, BMP-2 is sequestered enabling solid phase presentation of BMP-2. We demonstrate osteogenesis in vivo with ultra-low doses of BMP-2. Our protein technology enhances the biological activity of collagen without using complex chemistries or changing the manufacturing of the base material and so opens a pathway to clinical translation.<br />Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Manuel Salmeron-Sanchez reports financial support was provided by 10.13039/501100000853University of Glasgow.<br /> (© 2023 The Authors.)
Details
- Language :
- English
- ISSN :
- 2590-0064
- Volume :
- 20
- Database :
- MEDLINE
- Journal :
- Materials today. Bio
- Publication Type :
- Academic Journal
- Accession number :
- 37179535
- Full Text :
- https://doi.org/10.1016/j.mtbio.2023.100641