Establishment of bovine trophoblast stem cells.

Authors :: Wang Y
Ming H
Yu L
Li J
Zhu L
Sun HX
Pinzon-Arteaga CA
Wu J
Jiang Z
Source :: Cell reports [Cell Rep] 2023 May 30; Vol. 42 (5), pp. 112439. Date of Electronic Publication: 2023 May 04.
Publication Year :: 2023
Abstract: Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.<br />Competing Interests: Declaration of interests Z.J. and Y.W are co-inventors on US provisional patent application 63/413,798 relating to bTSCs and uses thereof.<br /> (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)