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Acute coronavirus infection triggers a TNF-dependent osteoporotic phenotype in mice.
- Source :
-
Life sciences [Life Sci] 2023 Jul 01; Vol. 324, pp. 121750. Date of Electronic Publication: 2023 May 02. - Publication Year :
- 2023
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Abstract
- Aims: Millions of people died during the COVID-19 pandemic, but the vast majority of infected individuals survived. Now, some consequences of the disease, known as long COVID, are been revealed. Although the respiratory system is the target of Sars-CoV-2, COVID-19 can influence other parts of the body, including bone. The aim of this work was to investigate the impact of acute coronavirus infection in bone metabolism.<br />Main Methods: We evaluated RANKL/OPG levels in serum samples of patients with and without acute COVID-19. In vitro, the effects of coronavirus in osteoclasts and osteoblasts were investigated. In vivo, we evaluated the bone phenotype in a BSL2 mouse model of SARS-like disease induced by murine coronavirus (MHV-3).<br />Key Findings: Patients with acute COVID-19 presented decreased OPG and increased RANKL/OPG ratio in the serum versus healthy individuals. In vitro, MHV-3 infected macrophages and osteoclasts, increasing their differentiation and TNF release. Oppositely, osteoblasts were not infected. In vivo, MHV-3 lung infection triggered bone resorption in the femur of mice, increasing the number of osteoclasts at 3dpi and decreasing at 5dpi. Indeed, apoptotic-caspase-3 <superscript>+</superscript> cells have been detected in the femur after infection as well as viral RNA. RANKL/OPG ratio and TNF levels also increased in the femur after infection. Accordingly, the bone phenotype of TNFRp55 <superscript>-/-</superscript> mice infected with MHV-3 showed no signs of bone resorption or increase in the number of osteoclasts.<br />Significance: Coronavirus induces an osteoporotic phenotype in mice dependent on TNF and on macrophage/osteoclast infection.<br />Competing Interests: Declaration of competing interest We declare no competing financial interest in relation to this work.<br /> (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Humans
Mice
Cell Differentiation
Osteoblasts
Osteoclasts metabolism
Osteoprotegerin metabolism
Pandemics
Phenotype
Post-Acute COVID-19 Syndrome
RANK Ligand metabolism
SARS-CoV-2 metabolism
Murine hepatitis virus metabolism
Murine hepatitis virus pathogenicity
Coronavirus Infections genetics
Coronavirus Infections metabolism
Bone Resorption metabolism
COVID-19 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0631
- Volume :
- 324
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 37142087
- Full Text :
- https://doi.org/10.1016/j.lfs.2023.121750