Back to Search
Start Over
Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) as an Indispensable Tool in Diagnostic Bacteriology: A Comparative Analysis With Conventional Technique.
- Source :
-
Cureus [Cureus] 2023 Mar 31; Vol. 15 (3), pp. e36984. Date of Electronic Publication: 2023 Mar 31 (Print Publication: 2023). - Publication Year :
- 2023
-
Abstract
- Introduction: Owing to its accurate diagnosis, rapid turnaround time, cost effectivity, and less rates of error, Matrix-assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) has replaced most of the phenotypic methods of identification. Thus, the objective of this study was to compare and evaluate MALDI-TOF MS to conventional biochemical-to identify bacterial microorganisms.<br />Methods: Different bacterial species isolated from 2010 to 2018 (pre-MALDI-TOF era), using routine bio-chemicals were compared to bacterial species isolated from 2019 to August 2021 (post MALDI-TOF), using MALDI-TOF, in the microbiology laboratory of a tertiary care hospital in North India. Chi-Square test (χ2) was used for the evaluation of bacterial identification between biochemical tests and MALDI-TOF MS association with a 95% confidence interval, considering wrong identification in genera or at a species level.<br />Results: Many different and new genera and species of bacteria could be identified using MALDI-TOF, which was not possible using only routine manual bio-chemicals like Kocuria rhizophilus , Rothia mucilaginosa,   Enterococcus casseliflavus, Enterococcus gallinarum, Leuconostoc, Leclercia adecarboxylata, Raoultella ornithological, Cryseobacterium indologenes. Conclusion: Each of the newly identified bacteria played an important role in deciding treatment. Wide use of the MALDI-TOF system will not only strengthen diagnostic stewardship but also encourage antimicrobial stewardship programs.<br />Competing Interests: The authors have declared that no competing interests exist.<br /> (Copyright © 2023, Gupta et al.)
Details
- Language :
- English
- ISSN :
- 2168-8184
- Volume :
- 15
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cureus
- Publication Type :
- Academic Journal
- Accession number :
- 37139282
- Full Text :
- https://doi.org/10.7759/cureus.36984