Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing.

Authors :: Merkert S
Haase A
Dahlmann J
Göhring G
Waqas FH
Pessler F
Martin U
Olmer R
Source :: Stem cell research [Stem Cell Res] 2023 Jun; Vol. 69, pp. 103090. Date of Electronic Publication: 2023 Apr 01.
Publication Year :: 2023
Abstract: The nuclear factor erythroid 2-related factor 2 (NFE2L2, known as NRF2) regulates the expression of antioxidative and anti-inflammatory proteins. In order to investigate its impact during viral infections and testing of antiviral compounds, we applied CRISPR/Cas9 editing to eliminate NRF2 in the human iPS cell line MHHi001-A and generated two NRF2 knockout iPSC clones MHHi001-A-6 and MHHi001-A-7. After differentiation into epithelia or endothelial cells, these cells are useful tools to examine the antiviral effects of activators of the NRF2 signaling pathway.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)