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Premature chromosome condensation--studies on human metastatic carcinoma cells.
- Source :
-
Human genetics [Hum Genet] 1986 May; Vol. 73 (1), pp. 44-52. - Publication Year :
- 1986
-
Abstract
- Utilizing the phenomenon of premature chromosome condensation (PCC) studies were carried out on interphase chromatin of metastatic cells from 52 cancerous effusions obtained from 45 patients presenting with various solid carcinomas. A highly individual pattern of distribution of the various interphase stages was detected, reflecting the heterogeneity of human solid tumors in an advanced stage. Nevertheless a variety of clinical, biologic, and technical factors were examined for their possible influence on these PCC patterns. The duration in culture was one of the influencing factors, as were the time lapse between the first diagnosis and the sampling of the respective effusion, or the nature of cytostatic therapy. Cytogenetic equivalents of gene amplification, as represented by "double minutes", could be found in the prematurely interphase chromatin of 35 of the 52 effusions. G1-PCC proved to be most reliable with regard to screening of double minutes. In addition, an adequate quality of Giemsa banding was achieved in PCC of 21 out of 24 effusions yielding a sufficient number of well-spread PCC. In six of these 21 cases PCC was superior to metaphase analysis in obtaining karyotypes, while the same was true for 14 of the 52 effusions screened for double minutes. Thus the PCC technique was shown to be an indispensable additional source of cytogenetic information in cells of human solid tumors.
- Subjects :
- Adult
Aged
Breast Neoplasms drug therapy
Breast Neoplasms genetics
Breast Neoplasms pathology
Carcinoma drug therapy
Carcinoma genetics
Carcinoma pathology
Chromosome Banding
Female
Humans
Karyotyping
Middle Aged
Neoplasm Metastasis
Neoplasms drug therapy
Neoplasms pathology
Ovarian Neoplasms drug therapy
Ovarian Neoplasms genetics
Ovarian Neoplasms pathology
Ploidies
Chromosome Aberrations drug effects
Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0340-6717
- Volume :
- 73
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Human genetics
- Publication Type :
- Academic Journal
- Accession number :
- 3710479
- Full Text :
- https://doi.org/10.1007/BF00292663