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Solid-Effect Dynamic Nuclear Polarization in Viscous Liquids at 9.4 T Using Narrow-Line Polarizing Agents.
- Source :
-
Journal of the American Chemical Society [J Am Chem Soc] 2023 May 10; Vol. 145 (18), pp. 10268-10274. Date of Electronic Publication: 2023 Apr 27. - Publication Year :
- 2023
-
Abstract
- Dynamic nuclear polarization (DNP) is a hyperpolarization method that is widely used for increasing the sensitivity of nuclear magnetic resonance (NMR) experiments. DNP is efficient in solid-state and liquid-state NMR, but its implementation in the intermediate state, namely, viscous media, is still less explored. Here, we show that a <superscript>1</superscript> H DNP enhancement of over 50 can be obtained in viscous liquids at a magnetic field of 9.4 T and a temperature of 315 K. This was accomplished by using narrow-line polarizing agents in glycerol, both the water-soluble α,γ-bisdiphenylen-β-phenylallyl (BDPA) and triarylmethyl radicals, and a microwave/RF double-resonance probehead. We observed DNP enhancements with a field profile indicative of the solid effect and investigated the influence of microwave power, temperature, and concentration on the <superscript>1</superscript> H NMR results. To demonstrate potential applications of this new DNP approach for chemistry and biology, we show hyperpolarized <superscript>1</superscript> H NMR spectra of tripeptides, triglycine, and glypromate, in glycerol- d <subscript>8</subscript> .
Details
- Language :
- English
- ISSN :
- 1520-5126
- Volume :
- 145
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 37104685
- Full Text :
- https://doi.org/10.1021/jacs.3c01358