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Phytochemical Estimation and Therapeutic Amelioration of Aesculus hippocastanum L. Seeds Ethanolic Extract in Gastric Ulcer in Rats Possibly by Inhibiting Prostaglandin Synthesis.
- Source :
-
Chinese journal of integrative medicine [Chin J Integr Med] 2023 Sep; Vol. 29 (9), pp. 818-824. Date of Electronic Publication: 2023 Apr 20. - Publication Year :
- 2023
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Abstract
- Objective: To quantify phytochemicals using liquid chromatography and mass spectroscopy (LCMS) analysis and explore the therapeutic effect of Aesculus hippocastanum L. (AH) seeds ethanolic extract against gastric ulcers in rats.<br />Methods: Preliminary phytochemical testing and LCMS analysis were performed according to standard methods. For treatment, the animals were divided into 7 groups including normal control, ulcer control, self-healing, AH seeds low and high doses, ranitidine and per se groups. Rats were orally administered with 10 mg/kg of indomethacin, excluding the normal control group (which received 1% carboxy methyl cellulose) and the per se group (received 200 mg/kg AH seeds extract). The test group rats were then given 2 doses of AH seeds extract (100 and 200 mg/kg, respectively), while the standard group was given ranitidine (50 mg/kg). On the 11th day, rats in all groups were sacrificed, and their stomach was isolated to calculate the ulcer index, and other parameters such as blood prostaglandin (PGE <subscript>2</subscript> ), tissue superoxide dismutase (SOD), catalase (CAT), malonyldialdehyde (MDA), and glutathione (GSH). All isolated stomach tissues were analyzed for histopathological findings.<br />Results: The phytochemical examination shows that the AH seeds contain alkaloids, flavonoids, saponins, phenolic components, and glycosides. LCMS analysis confirms the presence of quercetin and rutin. The AH seeds extract showed significant improvement in gastric mucosa conditions after indomethacin-induced gastric lesions (P<0.01). Further marked improvement in blood PGE <subscript>2</subscript> and antioxidant enzymes, SOD, CAT, MDA and GSH, were observed compared with self-healing and untreated ulcer-induced groups (P<0.01). Histopathology results confirmed that AH seeds extract improved the mucosal layer and gastric epithelial membrane in treated groups compared to untreated ulcer-induced groups.<br />Conclusions: LCMS report confirms the presence of quercetin and rutin in AH seeds ethanolic extract. The therapeutic effect of AH seeds extract against indomethacin-induced ulcer in rat model indicated the regenerated membrane integrity, with improved cellular functions and mucus thickness. Further, improved antioxidant enzyme level would help to reduce PGE <subscript>2</subscript> biosynthesis.<br /> (© 2023. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature.)
- Subjects :
- Rats
Animals
Antioxidants pharmacology
Antioxidants therapeutic use
Ranitidine adverse effects
Ulcer drug therapy
Quercetin
Plant Extracts pharmacology
Plant Extracts therapeutic use
Plant Extracts chemistry
Indomethacin therapeutic use
Glutathione
Superoxide Dismutase
Rutin adverse effects
Prostaglandins adverse effects
Phytochemicals therapeutic use
Stomach Ulcer chemically induced
Stomach Ulcer drug therapy
Stomach Ulcer pathology
Aesculus
Subjects
Details
- Language :
- English
- ISSN :
- 1993-0402
- Volume :
- 29
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Chinese journal of integrative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 37079159
- Full Text :
- https://doi.org/10.1007/s11655-023-3734-9