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Electro-enzymatic ATP regeneration coupled to biocatalytic phosphorylation reactions.
- Source :
-
Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2023 Aug; Vol. 152, pp. 108432. Date of Electronic Publication: 2023 Apr 04. - Publication Year :
- 2023
-
Abstract
- Adenosine-5-triphosphate (ATP) is the main energy vector in biological systems, thus its regeneration is an important issue for the application of many enzymes of interest in biocatalysis and synthetic biology. We have developed an electroenzymatic ATP regeneration system consisting in a gold electrode modified with a floating phospholipid bilayer that allows coupling the catalytic activity of two membrane-bound enzymes: NiFeSe hydrogenase from Desulfovibrio vulgaris and F <subscript>1</subscript> F <subscript>o</subscript> -ATP synthase from Escherichia coli. Thus, H <subscript>2</subscript> is used as a fuel for producing ATP. This electro-enzymatic assembly is studied as ATP regeneration system of phosphorylation reactions catalysed by kinases, such as hexokinase and NAD <superscript>+</superscript> -kinase for respectively producing glucose-6-phosphate and NADP <superscript>+</superscript> .<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1878-562X
- Volume :
- 152
- Database :
- MEDLINE
- Journal :
- Bioelectrochemistry (Amsterdam, Netherlands)
- Publication Type :
- Academic Journal
- Accession number :
- 37030092
- Full Text :
- https://doi.org/10.1016/j.bioelechem.2023.108432