The Biochemical Characteristics in Experimental Keratomycosis.

Authors :: Dong C
Huang Y
Source :: Current eye research [Curr Eye Res] 2023 Aug; Vol. 48 (8), pp. 691-698. Date of Electronic Publication: 2023 Jun 07.
Publication Year :: 2023
Abstract: Purpose: To investigate the biochemical characteristics in experimental keratomycosis.<br />Methods: Experimental mice were injected with Fusarium solanum solution Controls mice received liposomes containing phosphate-buffered saline (PBS-LIP). Raman spectroscopy was used to analyze the biochemical characteristics. The infiltration of inflammatory cells was analyzed by histopathology. Cytokine mRNA levels were detected by real-time polymerase chain reaction.<br />Results: Raman Spectroscopy: In the experimental group, collagen, lipids, amide I and III were decreased, amide II, hyper proline amino acids, and arginine were increased, and proline and phenylalanine were significantly increased on day 3. Histopathology: more severe keratomycosis developed in the experimental group than in the control group. Statistically significant mRNA expression of Collagen4\MMP2\MMP9\TIMP1.MMP9 was negatively correlated with the secretion of Collagen4.<br />Conclusions: Matrix metalloproteinases are involved in biochemical changes in keratomycosis.