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FIPRESCI: droplet microfluidics based combinatorial indexing for massive-scale 5'-end single-cell RNA sequencing.
- Source :
-
Genome biology [Genome Biol] 2023 Apr 06; Vol. 24 (1), pp. 70. Date of Electronic Publication: 2023 Apr 06. - Publication Year :
- 2023
-
Abstract
- Single-cell RNA sequencing methods focusing on the 5'-end of transcripts can reveal promoter and enhancer activity and efficiently profile immune receptor repertoire. However, ultra-high-throughput 5'-end single-cell RNA sequencing methods have not been described. We introduce FIPRESCI, 5'-end single-cell combinatorial indexing RNA-Seq, enabling massive sample multiplexing and increasing the throughput of the droplet microfluidics system by over tenfold. We demonstrate FIPRESCI enables the generation of approximately 100,000 single-cell transcriptomes from E10.5 whole mouse embryos in a single-channel experiment, and simultaneous identification of subpopulation differences and T cell receptor signatures of peripheral blood T cells from 12 cancer patients.<br /> (© 2023. The Author(s).)
Details
- Language :
- English
- ISSN :
- 1474-760X
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genome biology
- Publication Type :
- Academic Journal
- Accession number :
- 37024957
- Full Text :
- https://doi.org/10.1186/s13059-023-02893-1