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Proteomic analysis defines the interactome of telomerase in the protozoan parasite, Trypanosoma brucei .

Authors :
Davis JA
Reyes AV
Nitika
Saha A
Wolfgeher DJ
Xu SL
Truman AW
Li B
Chakrabarti K
Source :
Frontiers in cell and developmental biology [Front Cell Dev Biol] 2023 Mar 16; Vol. 11, pp. 1110423. Date of Electronic Publication: 2023 Mar 16 (Print Publication: 2023).
Publication Year :
2023

Abstract

Telomerase is a ribonucleoprotein enzyme responsible for maintaining the telomeric end of the chromosome. The telomerase enzyme requires two main components to function: the telomerase reverse transcriptase (TERT) and the telomerase RNA (TR), which provides the template for telomeric DNA synthesis. TR is a long non-coding RNA, which forms the basis of a large structural scaffold upon which many accessory proteins can bind and form the complete telomerase holoenzyme. These accessory protein interactions are required for telomerase activity and regulation inside cells. The interacting partners of TERT have been well studied in yeast, human, and Tetrahymena models, but not in parasitic protozoa, including clinically relevant human parasites. Here, using the protozoan parasite, Trypanosoma brucei ( T. brucei ) as a model, we have identified the interactome of T. brucei TERT ( Tb TERT) using a mass spectrometry-based approach. We identified previously known and unknown interacting factors of Tb TERT, highlighting unique features of T. brucei telomerase biology. These unique interactions with Tb TERT, suggest mechanistic differences in telomere maintenance between T. brucei and other eukaryotes.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2023 Davis, Reyes, Nitika, Saha, Wolfgeher, Xu, Truman, Li and Chakrabarti.)

Details

Language :
English
ISSN :
2296-634X
Volume :
11
Database :
MEDLINE
Journal :
Frontiers in cell and developmental biology
Publication Type :
Academic Journal
Accession number :
37009488
Full Text :
https://doi.org/10.3389/fcell.2023.1110423