CRISPR/LbCas12a-Mediated Genome Editing in Soybean.

Authors :: Liang D
Liu Y
Li C
Wen Q
Xu J
Geng L
Liu C
Jin H
Gao Y
Zhong H
Dawson J
Tian B
Barco B
Su X
Dong S
Li C
Elumalai S
Que Q
Jepson I
Shi L
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2653, pp. 39-52.
Publication Year :: 2023
Abstract: Currently methods for generating soybean edited lines are time-consuming, inefficient, and limited to certain genotypes. Here we describe a fast and highly efficient genome editing method based on CRISPR-Cas12a nuclease system in soybean. The method uses Agrobacterium-mediated transformation to deliver editing constructs and uses aadA or ALS genes as selectable marker. It only takes about 45 days to obtain greenhouse-ready edited plants at higher than 30% transformation efficiency and 50% editing rate. The method is applicable to other selectable markers including EPSPS and has low transgene chimera rate. The method is also genotype-flexible and has been applied to genome editing of several elite soybean varieties.<br /> (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)