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Cost-effective, scalable production of glucose oxidase using Casuarina equisetifolia biomass and its application in the bio-Fenton oxidation process for the removal of trace organic contaminants from wastewater.
- Source :
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Bioresource technology [Bioresour Technol] 2023 Jun; Vol. 377, pp. 128958. Date of Electronic Publication: 2023 Mar 24. - Publication Year :
- 2023
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Abstract
- This study focuses on using Casuarina equisetifolia biomass for pilot-scale glucose oxidase production from Aspergillus niger and its application in the removal of trace organic contaminants (TrOCs) from municipal wastewater through the bio-Fenton oxidation. The cost of glucose oxidase was 0.005 $/U, including the optimum production parameters, 10% biomass, 7% sucrose, 1% peptone, and 3% CaCO <subscript>3</subscript> at 96 h with an enzyme activity of 670 U/mL. Optimized conditions for H <subscript>2</subscript> O <subscript>2</subscript> were 1 M glucose, 100 U/mL glucose oxidase, and 120 mins of incubation, resulting in 544.3 mg/L H <subscript>2</subscript> O <subscript>2</subscript> . Thus, H <subscript>2</subscript> O <subscript>2</subscript> produced under these conditions lead to bio-Fenton oxidation resulting in the removal of 36-92% of nine TrOCs in municipal wastewater at pH 7.0 in 360 mins. Therefore, this work establishes the cost-effective glucose oxidase-producing H <subscript>2</subscript> O <subscript>2</subscript> as an attractive bioremediating agent to enhance the removal of TrOCs in wastewater at neutral pH.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 377
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 36965584
- Full Text :
- https://doi.org/10.1016/j.biortech.2023.128958