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A Plasmonic Fluor-Lightened Microneedle Array Enables Ultrasensitive Multitarget Whole Blood Diagnosis of Anemia in A Paper Origami-Based Device.
- Source :
-
Small (Weinheim an der Bergstrasse, Germany) [Small] 2023 Jun; Vol. 19 (26), pp. e2300464. Date of Electronic Publication: 2023 Mar 22. - Publication Year :
- 2023
-
Abstract
- This work reports a portable, origami-type paper device with a plasmonic fluor-labeled microneedle sensing module for the multiplexed quantification of anemia biomarkers in whole blood. Sequential steps, including serum separation, target enrichment, and multiplexed readout by a gel imager, are rapidly accomplished with the flexible and highly integrated device. The microneedle array enabled efficient sampling of trace targets from ng mL <superscript>-1</superscript> to pg mL <superscript>-1</superscript> level. Combined with the plasmonic fluor label, the signal is improved by ≈7.6 folds compared with the flat substrate-based assay. The device is applied to simultaneously quantify hemoglobin (Hb), ferritin, folic acid (FA), and vitamin B <subscript>12</subscript> (VB <subscript>12</subscript> ), which are four anemia biomarkers distributed in different environments with different concentration ranges. Featured by the small sample volume (150 µL), short assay time (20 min), low cost (2 $), robust stability, and user-friendliness, the device is promising for the rapid and accurate diagnosis of anemia in real practice.<br /> (© 2023 Wiley-VCH GmbH.)
- Subjects :
- Humans
Folic Acid
Vitamin B 12
Hemoglobins
Biomarkers
Anemia diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1613-6829
- Volume :
- 19
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Small (Weinheim an der Bergstrasse, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 36950741
- Full Text :
- https://doi.org/10.1002/smll.202300464