A fluorescence biosensor based on DNA aptamers-COF for highly selective detection of ATP and thrombin.

Authors :: Jouha J
Li F
Xiong H
Source :: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2023 Jul 05; Vol. 295, pp. 122615. Date of Electronic Publication: 2023 Mar 12.
Publication Year :: 2023
Abstract: Due to their distinctive physical, chemical, electrical, and optical properties as well as their prospective uses, 2D covalent organic framework (COF) have attracted much attention. Herein, TaTPA-COF was effectively synthesized from the condensation of TTA and TFPA using a facile solvothermal method and characterized by SEM image, FT-IR spectra, and PXRD pattern. The generated bulk TaTPA-COF materials combined with DNA aptamers are utilized as the acceptor (quencher) for the highly sensitive and selective detection of adenosine 5'-triphosphate (ATP) and thrombin, with a novel fluorescence biosensing platform and a proof-of-concept application.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 Elsevier B.V. All rights reserved.)

Subjects :: Thrombin
Spectroscopy, Fourier Transform Infrared
Adenosine Triphosphate
Aptamers, Nucleotide chemistry
Metal-Organic Frameworks chemistry
Biosensing Techniques methods

Language :: English
ISSN :: 1873-3557
Volume :: 295
Database :: MEDLINE
Journal :: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Publication Type :: Academic Journal
Accession number :: 36933442
Full Text :: https://doi.org/10.1016/j.saa.2023.122615

Full Text Access

Tools