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BARX1 promotes osteosarcoma cell proliferation and invasion by regulating HSPA6 expression.
- Source :
-
Journal of orthopaedic surgery and research [J Orthop Surg Res] 2023 Mar 16; Vol. 18 (1), pp. 211. Date of Electronic Publication: 2023 Mar 16. - Publication Year :
- 2023
-
Abstract
- Osteosarcoma (OS) is a bone tumour affecting adolescents. Dysregulation of Barx homeobox 1 (BARX1) expression is involved in various cancers, but its function and mechanism in the process of OS are undefined. This study revealed that BARX1 expression is higher in OS tissue than in adjacent normal tissue. Downregulation of BARX1 in OS cells significantly suppressed their proliferation and migration, whereas enforced expression of exogenous BARX1 exerted the opposite effects on OS cells. Subsequently, heat shock 70-kDa protein 6 (HSPA6) expression was clearly increased after BARX1 overexpression in OS cells, as confirmed by RNA sequencing. The dual-luciferase reporter assay confirmed that HSPA6 expression is directly regulated by BARX1. The in vitro assay indicated that silencing HSPA6 expression attenuated OS proliferation and migration induced by BARX1. A dual immunofluorescence labelling assay provided further evidence that BARX1 was overexpressed and associated with HSPA6 overexpression in OS tumour tissue. In conclusion, BARX1 promotes OS cell proliferation and migration by inducing the expression of HSPA6, which plays an oncogenic role in OS. BARX1 and HSPA6 can potentially act as novel therapeutic targets for OS.<br /> (© 2023. The Author(s).)
- Subjects :
- Adolescent
Humans
Cell Movement genetics
Cell Line, Tumor
Gene Expression Regulation, Neoplastic genetics
Cell Proliferation genetics
Transcription Factors genetics
Homeodomain Proteins genetics
Homeodomain Proteins metabolism
MicroRNAs genetics
Osteosarcoma pathology
Bone Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1749-799X
- Volume :
- 18
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of orthopaedic surgery and research
- Publication Type :
- Academic Journal
- Accession number :
- 36927457
- Full Text :
- https://doi.org/10.1186/s13018-023-03690-z