Back to Search
Start Over
Primary structure determination of seven novel N-linked carbohydrate chains derived from hemocyanin of Lymnaea stagnalis. 3-O-methyl-D-galactose and N-acetyl-D-galactosamine as constituents of xylose-containing N-linked oligosaccharides in an animal glycoprotein.
- Source :
-
European journal of biochemistry [Eur J Biochem] 1987 Dec 01; Vol. 169 (2), pp. 399-411. - Publication Year :
- 1987
-
Abstract
- Hemocyanin from the freshwater snail Lymnaea stagnalis is a high-molecular-mass copper-containing glyco-protein which functions as oxygen carrier in the hemolymph. To release the carbohydrate chains, the protein was digested by pronase followed by hydrazinolysis and reduction. The oligosaccharide-alditols were purified by gel permeation chromatography on Bio-Gel P-4, followed by HPLC on a Lichrosorb-NH2 column. Using 500-MHz 1H-NMR spectroscopy, in conjunction with sugar, methylation and deamination analysis, the following seven novel primary oligosaccharide structures could be unravelled. (Formula: see text).
- Subjects :
- Animals
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Indicators and Reagents
Magnetic Resonance Spectroscopy methods
Molecular Sequence Data
Acetylgalactosamine analysis
Galactosamine analogs & derivatives
Hemocyanins
Lymnaea
Methylgalactosides analysis
Methylglycosides analysis
Oligosaccharides isolation & purification
Xylose analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2956
- Volume :
- 169
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 3691499
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1987.tb13626.x