Mycobacterium tuberculosis retains viability in RNAlater buffer but not in GTC-TCEP and DNA/RNA Shield.

Authors :: Krausser L
Braet SM
Benaamar Z
Van Dyck-Lippens M
de Jong BC
Rigouts L
Source :: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2023 May; Vol. 106 (1), pp. 115905. Date of Electronic Publication: 2023 Feb 01.
Publication Year :: 2023
Abstract: Efficient inactivation of clinical samples containing mycobacteria is crucial for biosafety during shipment and handling. When stored in RNAlater, Mycobacterium tuberculosis H37Ra remains viable, and our results suggest that at -20 °C and 4 °C changes in the mycobacterial transcriptome are possible. Only GTC-TCEP and DNA/RNA Shield inactivate sufficiently for shipment.<br />Competing Interests: Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

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