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Antibody development to identify components of IIS and mTOR signaling pathways in lepidopteran species, a set of non-model insects.
- Source :
-
MicroPublication biology [MicroPubl Biol] 2023 Feb 16; Vol. 2023. Date of Electronic Publication: 2023 Feb 16 (Print Publication: 2023). - Publication Year :
- 2023
-
Abstract
- Nutritional stress impacts many insect species that have differing reproductive strategies and life histories, yet it is unclear how nutrient-sensing signaling pathways mediate tissue-specific responses to changes in dietary input. In Drosophila melanogaster , insulin/insulin-like growth factor (IIS) and mTOR-mediated signaling within adipocytes regulates oogenesis. To facilitate comparative study of nutrient-sensing pathway activity in the fat body, we developed antibodies to assess IIS (anti-FOXO) and mTOR signaling (anti-TOR) across three nymphalid species (Lepidoptera). By optimizing whole-mount fat body immunostaining, we find FOXO nuclear enrichment in adult adipocytes, like that observed in Drosophila . Additionally, we show a previously uncharacterized TOR localization pattern in the fat body.<br /> (Copyright: © 2023 by the authors.)
Details
- Language :
- English
- ISSN :
- 2578-9430
- Volume :
- 2023
- Database :
- MEDLINE
- Journal :
- MicroPublication biology
- Publication Type :
- Academic Journal
- Accession number :
- 36879981
- Full Text :
- https://doi.org/10.17912/micropub.biology.000755