SARS-CoV-2 immune complex triggers human monocyte necroptosis.

Authors :: Santos LD
Antunes KH
Cassão G
Gonçalves JI
Abbadi BL
Bizarro CV
Basso LA
Machado P
de Souza APD
Porto BN
Source :: International immunopharmacology [Int Immunopharmacol] 2023 Apr; Vol. 117, pp. 109954. Date of Electronic Publication: 2023 Feb 27.
Publication Year :: 2023
Abstract: We analyzed the ability of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) itself and SARS-CoV-2-IgG immune complexes to trigger human monocyte necroptosis. SARS-CoV-2 was able to induce monocyte necroptosis dependently of MLKL activation. Necroptosis-associated proteins (RIPK1, RIPK3 and MLKL) were involved in SARS-CoV-2N1 gene expression in monocytes. SARS-CoV-2 immune complexes promoted monocyte necroptosis in a RIPK3- and MLKL-dependent manner, and Syk tyrosine kinase was necessary for SARS-CoV-2 immune complex-induced monocyte necroptosis, indicating the involvement of Fcγ receptors on necroptosis. Finally, we provide evidence that elevated LDH levels as a marker of lytic cell death are associated with COVID-19 pathogenesis.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2023 Elsevier B.V. All rights reserved.)