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A procedure in mice to obtain intact pituitary-infundibulum-hypothalamus preparations: a method to evaluate the reconstruction of hypothalamohypophyseal system.
- Source :
-
Pituitary [Pituitary] 2023 Apr; Vol. 26 (2), pp. 197-208. Date of Electronic Publication: 2023 Mar 02. - Publication Year :
- 2023
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Abstract
- Purpose: The histopathological study of brain tissue is a common method in neuroscience. However, efficient procedures to preserve the intact hypothalamic-pituitary brain specimens are not available in mice for histopathological study.<br />Method: We describe a detailed procedure for obtaining mouse brain with pituitary-hypothalamus continuity. Unlike the traditional methods, we collect the brain via a ventral approach. We cut the intraoccipital synchondrosis, transection the endocranium of pituitary, broke the spheno-occipital synchondrosis, expose the posterior edge of pituitary, separate the trigeminal nerve, then the intact pituitary gland was preserved.<br />Result: We report an more effective and practical method to obtain continuous hypothalamus -pituitary preparations based on the preserve of leptomeninges.<br />Compared With the Existing Methods: Our procedure effectively protects the integrity of the fragile infundibulum preventing the pituitary from separating from the hypothalamus. This procedure is more convenient and efficient.<br />Conclusion: We present a convenient and practical procedure to obtain intact hypothalamic-pituitary brain specimens for subsequent histopathological evaluation in mice.<br /> (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Details
- Language :
- English
- ISSN :
- 1573-7403
- Volume :
- 26
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Pituitary
- Publication Type :
- Academic Journal
- Accession number :
- 36862266
- Full Text :
- https://doi.org/10.1007/s11102-023-01299-3