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In vitro expansion of Wilms' tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells.
- Source :
-
STAR protocols [STAR Protoc] 2023 Mar 17; Vol. 4 (1), pp. 102053. Date of Electronic Publication: 2023 Jan 30. - Publication Year :
- 2023
-
Abstract
- Wilms' tumor protein 1 (WT1) is a tumor-associated antigen overexpressed in various cancers. As a self-antigen, negative selection reduces the number of WT1-specific T cell receptors (TCRs). Here, we provide a protocol to generate WT1 <subscript>37-45</subscript> -specific TCRs using healthy human peripheral blood mononuclear cells. We describe the expansion of WT1-specific T cell clones by two consecutive in vitro stimulations with autologous WT1 <subscript>37-45</subscript> -pulsed dendritic cells and peripheral blood lymphocytes. We then detail the detection with human leukocyte antigen/WT1 <subscript>37-45</subscript> tetramers.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 4
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 36853720
- Full Text :
- https://doi.org/10.1016/j.xpro.2023.102053