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Mesenchymal stem cell-derived neural progenitors attenuate proinflammatory microglial activation via paracrine mechanisms.
- Source :
-
Regenerative medicine [Regen Med] 2023 Mar; Vol. 18 (3), pp. 259-273. Date of Electronic Publication: 2023 Feb 27. - Publication Year :
- 2023
-
Abstract
- Background: Mesenchymal stem cell-derived neural progenitor cell (MSC-NP) therapy is an experimental approach to treat multiple sclerosis. The influence of MSC-NPs on microglial activation was investigated. Methods: Microglia were stimulated in the presence of MSC-NP-conditioned media, and proinflammatory or proregenerative marker expression was assessed by quantitative PCR and ELISA. Results: Microglia stimulated in the presence of MSC-NP-conditioned media displayed reduced expression of proinflammatory markers including CCL2, increased expression of proregenerative markers and reduced phagocytic activity. The paracrine effects of MSC-NPs from multiple donors correlated with TGF-β3 gene expression and was reversed by TGF-β signaling inhibition. Conclusion: MSC-NPs promote beneficial microglial polarization through secreted factors. This study suggests that microglia are a potential therapeutic target of MSC-NP cell therapy.
Details
- Language :
- English
- ISSN :
- 1746-076X
- Volume :
- 18
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Regenerative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 36852422
- Full Text :
- https://doi.org/10.2217/rme-2023-0005