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RBD and Spike DNA-Based Immunization in Rabbits Elicited IgG Avidity Maturation and High Neutralizing Antibody Responses against SARS-CoV-2.

Authors :
da Costa HHM
Orts DJB
Moura AD
Duarte-Neto AN
Cirqueira CS
Réssio RA
Kanamura CT
Miguita K
Ferreira JE
Santos RTM
Adriani PP
Cunha-Junior JP
Astray RM
Catarino RM
Lancelotti M
Prudencio CR
Source :
Viruses [Viruses] 2023 Feb 17; Vol. 15 (2). Date of Electronic Publication: 2023 Feb 17.
Publication Year :
2023

Abstract

Neutralizing antibodies (nAbs) are a critical part of coronavirus disease 2019 (COVID-19) research as they are used to gain insight into the immune response to severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) infections. Among the technologies available for generating nAbs, DNA-based immunization methods are an alternative to conventional protocols. In this pilot study, we investigated whether DNA-based immunization by needle injection in rabbits was a viable approach to produce a functional antibody response. We demonstrated that three doses of DNA plasmid carrying the gene encoding the full-length spike protein (S) or the receptor binding domain (RBD) of SARS-CoV-2 induced a time-dependent increase in IgG antibody avidity maturation. Moreover, the IgG antibodies displayed high cross neutralization by live SARS-CoV-2 and pseudoviruses neutralization assays. Thus, we established a simple, low cost and feasible DNA-based immunization protocol in rabbits that elicited high IgG avidity maturation and nAbs production against SARS-CoV-2, highlighting the importance of DNA-based platforms for developing new immunization strategies against SARS-CoV-2 and future emerging epidemics.

Details

Language :
English
ISSN :
1999-4915
Volume :
15
Issue :
2
Database :
MEDLINE
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
36851769
Full Text :
https://doi.org/10.3390/v15020555