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Rapid detection of three mycotoxins in animal feed materials using competitive ELISA-based origami microfluidic paper analytical device (μPAD).

Authors :
Feng S
Hua MZ
Roopesh MS
Lu X
Source :
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2023 Apr; Vol. 415 (10), pp. 1943-1951. Date of Electronic Publication: 2023 Feb 27.
Publication Year :
2023

Abstract

We report the development of a competitive ELISA-based origami microfluidic paper-based analytical device (μPAD) for the detection of mycotoxins in animal feed material. The μPAD was patterned using the wax printing technique with the design of a testing pad in the middle and two absorption pads at the side. Anti-mycotoxin antibodies were effectively immobilized on chitosan-glutaraldehyde-modified sample reservoirs in the μPAD. The determination of zearalenone, deoxynivalenol, and T-2 toxin in corn flour was successfully achieved by performing competitive ELISA on the μPAD in 20 min. Colorimetric results were easily distinguished by the naked eye with a detection limit of 1 µg/mL for all three mycotoxins. The μPAD integrated with competitive ELISA holds potential for practical applications in the livestock industry for rapid, sensitive, and cost-effective detection of different mycotoxins in animal feed materials.<br /> (© 2023. Springer-Verlag GmbH Germany, part of Springer Nature.)

Details

Language :
English
ISSN :
1618-2650
Volume :
415
Issue :
10
Database :
MEDLINE
Journal :
Analytical and bioanalytical chemistry
Publication Type :
Academic Journal
Accession number :
36847793
Full Text :
https://doi.org/10.1007/s00216-023-04612-y