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Mitochondrial phosphatidylethanolamine modulates UCP1 to promote brown adipose thermogenesis.

Authors :
Johnson JM
Peterlin AD
Balderas E
Sustarsic EG
Maschek JA
Lang MJ
Jara-Ramos A
Panic V
Morgan JT
Villanueva CJ
Sanchez A
Rutter J
Lodhi IJ
Cox JE
Fisher-Wellman KH
Chaudhuri D
Gerhart-Hines Z
Funai K
Source :
Science advances [Sci Adv] 2023 Feb 24; Vol. 9 (8), pp. eade7864. Date of Electronic Publication: 2023 Feb 24.
Publication Year :
2023

Abstract

Thermogenesis by uncoupling protein 1 (UCP1) is one of the primary mechanisms by which brown adipose tissue (BAT) increases energy expenditure. UCP1 resides in the inner mitochondrial membrane (IMM), where it dissipates membrane potential independent of adenosine triphosphate (ATP) synthase. Here, we provide evidence that phosphatidylethanolamine (PE) modulates UCP1-dependent proton conductance across the IMM to modulate thermogenesis. Mitochondrial lipidomic analyses revealed PE as a signature molecule whose abundance bidirectionally responds to changes in thermogenic burden. Reduction in mitochondrial PE by deletion of phosphatidylserine decarboxylase (PSD) made mice cold intolerant and insensitive to β3 adrenergic receptor agonist-induced increase in whole-body oxygen consumption. High-resolution respirometry and fluorometry of BAT mitochondria showed that loss of mitochondrial PE specifically lowers UCP1-dependent respiration without compromising electron transfer efficiency or ATP synthesis. These findings were confirmed by a reduction in UCP1 proton current in PE-deficient mitoplasts. Thus, PE performs a previously unknown role as a temperature-responsive rheostat that regulates UCP1-dependent thermogenesis.

Details

Language :
English
ISSN :
2375-2548
Volume :
9
Issue :
8
Database :
MEDLINE
Journal :
Science advances
Publication Type :
Academic Journal
Accession number :
36827367
Full Text :
https://doi.org/10.1126/sciadv.ade7864