Increased IL-1α expression is correlated with bladder cancer malignant progression.

Introduction: To explore the function of interleukin 1α (IL-1α) in bladder cancer (BCa).
Material and Methods: Immunohistochemistry (IHC) was used to test the protein expression of IL-1α in BCa tissues. The relationship between IL-1α and clinical characteristics was analyzed by the Kaplan-Meier curve method. The gene and protein expression was tested by reverse transcription quantitative polymerase chain reaction (RT-q-PCR) and western blot, respectively. Colony formation and MTT assays were used to detect the potential of proliferation in vitro , and scratch and transwell chamber assays were used to detect the potential of invasion in vitro . Markers of proliferation such as Ki-67 and proliferating cell nuclear antigen (PCNA) and markers of invasion such as MMP-2 and MMP-9 were detected by western blot. Xenograft study was used for the in vivo experiment.
Results: We found that IL-1α was highly expressed in BCa patients while highly expressed IL-1α was significantly related to short overall survival and progression-free survival in BCa as well. Moreover, knockdown of IL-1α might inhibit the ability of cancer cells to proliferate and invade or migrate both in vitro and in vivo .
Conclusions: Our findings suggested that IL-1α might be a therapy target for BCa malignant progression.
Competing Interests: The authors declare no conflict of interest.
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