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Determination of the Chromatin Openness in Bacterial Genomes.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2611, pp. 63-69. - Publication Year :
- 2023
-
Abstract
- The hyperactive Tn5 transposase in the ATAC-seq method has been widely used to determine the open DNA regions and understand the overall epigenomic regulation in the chromatins of eukaryotic cells. Here, we describe POP-seq (Prokaryotic chromatin Openness Profiling sequencing), an adaptation of the ATAC-seq method, to interrogate changes in the openness of prokaryotic nucleoids.<br /> (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2611
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 36807064
- Full Text :
- https://doi.org/10.1007/978-1-0716-2899-7_5