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Label-free single cell proteomics utilizing ultrafast LC and MS instrumentation: A valuable complementary technique to multiplexing.
- Source :
-
Proteomics [Proteomics] 2023 Jul; Vol. 23 (13-14), pp. e2200162. Date of Electronic Publication: 2023 Mar 01. - Publication Year :
- 2023
-
Abstract
- The ability to map a proteomic fingerprint to transcriptomic data would master the understanding of how gene expression translates into actual phenotype. In contrast to nucleic acid sequencing, in vitro protein amplification is impossible and no single cell proteomic workflow has been established as gold standard yet. Advances in microfluidic sample preparation, multi-dimensional sample separation, sophisticated data acquisition strategies, and intelligent data analysis algorithms have resulted in major improvements to successfully analyze such tiny sample amounts with steadily boosted performance. However, among the broad variation of published approaches, it is commonly accepted that highest possible sensitivity, robustness, and throughput are still the most urgent needs for the field. While many labs have focused on multiplexing to achieve these goals, label-free SCP is a highly promising strategy as well whenever high dynamic range and unbiased accurate quantification are needed. We here focus on recent advances in label-free single-cell mass spectrometry workflows and try to guide our readers to choose the best method or combinations of methods for their specific applications. We further highlight which techniques are most propitious in the future and which applications but also limitations we foresee for the field.<br /> (© 2023 The Authors. Proteomics published by Wiley-VCH GmbH.)
- Subjects :
- Mass Spectrometry methods
Proteome analysis
Proteomics methods
Algorithms
Subjects
Details
- Language :
- English
- ISSN :
- 1615-9861
- Volume :
- 23
- Issue :
- 13-14
- Database :
- MEDLINE
- Journal :
- Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 36806919
- Full Text :
- https://doi.org/10.1002/pmic.202200162