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Detection of multiple biomarkers associated with satellite cell fate in the contused skeletal muscle of rats for wound age estimation.
- Source :
-
International journal of legal medicine [Int J Legal Med] 2023 May; Vol. 137 (3), pp. 875-886. Date of Electronic Publication: 2023 Feb 17. - Publication Year :
- 2023
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Abstract
- From the perspective of forensic wound age estimation, experiments related to skeletal muscle regeneration after injury have rarely been reported. Here, we examined the time-dependent expression patterns of multiple biomarkers associated with satellite cell fate, including the transcription factor paired box 7 (Pax7), myoblast determination protein (MyoD), myogenin, and insulin-like growth factor (IGF-1), using immunohistochemistry, western blotting, and quantitative real-time PCR in contused skeletal muscle. An animal model of skeletal muscle contusion was established in 30 Sprague-Dawley male rats, and another five rats were employed as non-contused controls. Morphometrically, the data obtained from the numbers of Pax7 + , MyoD + , and myogenin + cells were highly correlated with the wound age. Pax7, MyoD, myogenin, and IGF-1 expression patterns were upregulated after injury at both the mRNA and protein levels. Pax7, MyoD, and myogenin protein expression levels confirmed the results of the morphometrical analysis. Additionally, the relative quantity of IGF-1 protein > 0.92 suggested a wound age of 3 to 7 days. The relative quantity of Pax7 mRNA > 2.44 also suggested a wound age of 3 to 7 days. Relative quantities of Myod1, Myog, and Igf1 mRNA expression > 2.78, > 7.80, or > 3.13, respectively, indicated a wound age of approximately 3 days. In conclusion, the expression levels of Pax7, MyoD, myogenin, and IGF-1 were upregulated in a time-dependent manner during skeletal muscle wound healing, suggesting the potential for using them as candidate biomarkers for wound age estimation in skeletal muscle.<br /> (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Subjects :
- Rats
Animals
Male
Myogenin genetics
Myogenin metabolism
Insulin-Like Growth Factor I genetics
Insulin-Like Growth Factor I metabolism
Rats, Sprague-Dawley
Muscle, Skeletal metabolism
Biomarkers metabolism
RNA, Messenger metabolism
MyoD Protein genetics
MyoD Protein metabolism
Contusions metabolism
Satellite Cells, Skeletal Muscle metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1437-1596
- Volume :
- 137
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of legal medicine
- Publication Type :
- Academic Journal
- Accession number :
- 36797435
- Full Text :
- https://doi.org/10.1007/s00414-023-02971-w