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Finding a vocation for validation: taking proteomics beyond association and location.
- Source :
-
RSC chemical biology [RSC Chem Biol] 2022 Dec 02; Vol. 4 (2), pp. 110-120. Date of Electronic Publication: 2022 Dec 02 (Print Publication: 2023). - Publication Year :
- 2022
-
Abstract
- First established in the seventies, proteomics, chemoproteomics, and most recently, spatial/proximity-proteomics technologies have empowered researchers with new capabilities to illuminate cellular communication networks that govern sophisticated decision-making processes. With an ever-growing inventory of these advanced proteomics tools, the onus is upon the researchers to understand their individual advantages and limitations, such that we can ensure rigorous implementation and conclusions derived from critical data interpretations backed up by orthogonal series of functional validations. This perspective-based on the authors' experience in applying varied proteomics workflows in complex living models-underlines key book-keeping considerations, comparing and contrasting most-commonly-deployed modern proteomics profiling technologies. We hope this article stimulates thoughts among expert users and equips new-comers with practical knowhow of what has become an indispensable tool in chemical biology, drug discovery, and broader life-science investigations.<br />Competing Interests: Small-molecule inhibitors derived from applications of REX technologies have been filed for patent.<br /> (This journal is © The Royal Society of Chemistry.)
Details
- Language :
- English
- ISSN :
- 2633-0679
- Volume :
- 4
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- RSC chemical biology
- Publication Type :
- Academic Journal
- Accession number :
- 36794020
- Full Text :
- https://doi.org/10.1039/d2cb00214k