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[Detection of IgG protein in human urine based on vertical flow paper microfluidic chip].
- Source :
-
Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2023 Jan 25; Vol. 39 (1), pp. 337-346. - Publication Year :
- 2023
-
Abstract
- The kidney is the body's most important organ and the protein components in urine could be detected for diagnosing certain diseases. The amount of IgG protein in urine could be used to determine the degree of kidney function damage. IgG protein in human urine was detected by vertical flow paper-based microfluidic chip, double-antibody sandwich immunoreaction, and cell phone image processing. The results showed that using an IgG antibody concentration of 500 μg/mL and a gold standard antibody concentration of 100 μg/mL, the image signal showed a good linear relationship in the range of IgG concentration of 0.2-3.2 μg/mL, with R <superscript>2</superscript> =0.973 3 achieved. A complete set of detection devices were designed and the detection method showed good non-specificity.
- Subjects :
- Humans
Immunoglobulin G
Kidney
Microfluidics
Microfluidic Analytical Techniques
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1872-2075
- Volume :
- 39
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 36738220
- Full Text :
- https://doi.org/10.13345/j.cjb.220246