Engineering a Yellow Thermostable Fluorescent Protein by Rational Design.

Authors :: Anderson MR
Padgett CM
Dargatz CJ
Nichols CR
Vittalam KR
DeVore NM
Source :: ACS omega [ACS Omega] 2022 Dec 28; Vol. 8 (1), pp. 436-443. Date of Electronic Publication: 2022 Dec 28 (Print Publication: 2023).
Publication Year :: 2022
Abstract: Thermal green protein (TGP) is an extremely stable, highly soluble synthetic green fluorescent protein. The quantum yield of TGP is lower than the closest related natural fluorescent protein, monomeric Azami-Green. We improved the thermal recovery of TGP through the introduction of a chromophore mutation, Q66E. Furthermore, we developed a yellow thermal protein (YTP) via mutation of histidine 193 to tyrosine. Incorporation of Q66E into YTP (YTP-E) improved chemostability and pH stability. Both YTP and YTP-E have superior thermostability compared to TGP or TGP-E. These proteins offer a new option for green or yellow fluorescence under harsh chemical or thermal conditions.<br />Competing Interests: The authors declare no competing financial interest.<br /> (© 2022 The Authors. Published by American Chemical Society.)