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Specific suppression of long terminal repeat retrotransposon mobilization in plants.
- Source :
-
Plant physiology [Plant Physiol] 2023 Apr 03; Vol. 191 (4), pp. 2245-2255. - Publication Year :
- 2023
-
Abstract
- The tissue culture passage necessary for the generation of transgenic plants induces genome instability. This instability predominantly involves the uncontrolled mobilization of LTR retrotransposons (LTR-TEs), which are the most abundant class of mobile genetic elements in plant genomes. Here, we demonstrate that in conditions inductive for high LTR-TE mobilization, like abiotic stress in Arabidopsis (Arabidopsis thaliana) and callus culture in rice (Oryza sativa), application of the reverse transcriptase (RT) inhibitor known as Tenofovir substantially affects LTR-TE RT activity without interfering with plant development. We observed that Tenofovir reduces extrachromosomal DNA accumulation and prevents new genomic integrations of the active LTR-TE ONSEN in heat-stressed Arabidopsis seedlings, and transposons of O. sativa 17 and 19 (Tos17 and Tos19) in rice calli. In addition, Tenofovir allows the recovery of plants free from new LTR-TE insertions. We propose the use of Tenofovir as a tool for studies of LTR-TE transposition and for limiting genetic instabilities of plants derived from tissue culture.<br />Competing Interests: Conflict of interest statement. None declared.<br /> (© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 191
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 36583226
- Full Text :
- https://doi.org/10.1093/plphys/kiac605