Chemical proteomic analysis of bile acid-protein targets in Enterococcus faecium .

Authors :: Yang X
Zhao X
Chen V
Hang HC
Source :: RSC chemical biology [RSC Chem Biol] 2022 Sep 20; Vol. 3 (12), pp. 1397-1402. Date of Electronic Publication: 2022 Sep 20 (Print Publication: 2022).
Publication Year :: 2022
Abstract: Bile acids are important gut microbiota metabolites that regulate both host and microbial functions. To identify the direct protein targets of bile acids in Enterococcus , we synthesized and validated the activity of a lithocholic acid (LCA) photoaffinity reporter, x-alk-LCA-3. Chemical proteomics of x-alk-LCA-3 in E. faecium Com15 reveals many candidate LCA-interacting proteins, which are involved in cell well synthesis, transcriptional regulation and metabolism. To validate the utility of bile acid photoaffinity labeling, we characterized a putative bile salt hydrolase (BSH) crosslinked by x-alk-LCA-3, and demonstrated that this BSH was effective in converting taurolithocholic acid (TLCA) to LCA in E. faecium and in vitro . Chemical proteomics should afford new opportunities to characterize bile acid-protein targets and mechanisms of action in the future.<br />Competing Interests: There are no conflicts to declare.<br /> (This journal is © The Royal Society of Chemistry.)

Full Text Access

Tools