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The O 6 -methylguanine-DNA methyltransferase (MGMT) promoter methylation status and clinical outcomes of Ewing sarcoma patients treated with irinotecan and temozolomide.
- Source :
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Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology [Rep Pract Oncol Radiother] 2022 Oct 31; Vol. 27 (5), pp. 759-767. Date of Electronic Publication: 2022 Oct 31 (Print Publication: 2022). - Publication Year :
- 2022
-
Abstract
- Background: There remains an unmet need to identify molecular biomarkers in Ewing sarcoma (ES). We sought to assess the influence of the O <superscript>6</superscript> -methylguanine-DNA methyltransferase ( MGMT ) promoter methylation on response and progression-free survival (PFS) following initiation of irinotecan and temozolomide (IT), PFS following initiation of vincristine, doxorubicin, and cyclophosphamide alternating with ifosfamide and etoposide (VDC-IE), and overall survival (OS).<br />Materials and Methods: Data of advanced ES patients, treated with IT were retrospectively collected. Patients were required to have progression after prior VDC-IE. MGMT promoter methylation was assessed on non-decalcified Formalin-fixed paraffin embedded (FFPE) tissue using methylation sensitive restriction enzyme-quantitative PCR (MSRE-qPCR). Survival was estimated by the Kaplan-Meier method.<br />Results: A total of 20 ES patients underwent MGMT promoter methylation testing, and were eligible for analysis. Five patients (25%) had methylated MGMT , whereas the remaining (15; 75%) had unmethylated promoter. Five (25%) had objective response to IT, with no observed difference by promoter methylation (p = 0.76). Median PFS from initiation of IT for methylated vs . unmethylated MGMT patients was 4.9 and 1.2 months, respectively, p = 0.69. Median PFS from date of initiation of VDC-IE was significantly superior in the methylated group; 27.8 vs . 8.6 months, p = 0.034. Median OS was superior but not statistically significant in the methylated group.<br />Conclusion: MGMT -promoter methylation did not correlate with clinical activity or outcomes following the IT regimen for advanced ES. However, methylated MGMT predicted significantly superior PFS following initiation of the standard VDC-IE protocol.<br />Competing Interests: Conflict of interest None to declare.<br /> (© 2022 Greater Poland Cancer Centre.)
Details
- Language :
- English
- ISSN :
- 1507-1367
- Volume :
- 27
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology
- Publication Type :
- Academic Journal
- Accession number :
- 36523794
- Full Text :
- https://doi.org/10.5603/RPOR.a2022.0084