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Inhibition of alkaline phosphatase activity and D-glucose uptake in rat renal brush-border membrane vesicles by aminoglycosides.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1987 Sep 18; Vol. 903 (1), pp. 31-6. - Publication Year :
- 1987
-
Abstract
- The binding of aminoglycoside antibiotics to, and their effects on, the plasma membrane were studied using isolated rat renal brush-border membrane vesicles. Dibekacin was noted to bind with brush-border membrane vesicles having a single class of many binding sites. 3H-labeled dibekacin binding was inhibited competitively by unlabeled dibekacin, gentamicin or amikacin. The inhibition constants obtained from the Dixon plots followed the order of gentamicin approximately equal to dibekacin greater than amikacin. The alkaline phosphatase activity of brush-border membrane vesicles was inhibited by gentamicin significantly, as was also observed by a histochemical study. Sodium-dependent D-glucose uptake by brush-border membrane vesicles was significantly inhibited by the addition of gentamicin.
- Subjects :
- Amikacin metabolism
Animals
Anti-Bacterial Agents metabolism
Binding, Competitive
Biological Transport drug effects
Dibekacin metabolism
Gentamicins metabolism
Gentamicins pharmacology
Histocytochemistry
Kidney drug effects
Male
Microvilli metabolism
Rats
Rats, Inbred Strains
Sodium pharmacology
Alkaline Phosphatase antagonists & inhibitors
Anti-Bacterial Agents pharmacology
Glucose metabolism
Kidney metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 903
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 3651455
- Full Text :
- https://doi.org/10.1016/0005-2736(87)90152-0