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VASH1-SVBP and VASH2-SVBP generate different detyrosination profiles on microtubules.

Authors :
Ramirez-Rios S
Choi SR
Sanyal C
Blum TB
Bosc C
Krichen F
Denarier E
Soleilhac JM
Blot B
Janke C
Stoppin-Mellet V
Magiera MM
Arnal I
Steinmetz MO
Moutin MJ
Source :
The Journal of cell biology [J Cell Biol] 2023 Feb 06; Vol. 222 (2). Date of Electronic Publication: 2022 Dec 13.
Publication Year :
2023

Abstract

The detyrosination/tyrosination cycle of α-tubulin is critical for proper cell functioning. VASH1-SVBP and VASH2-SVBP are ubiquitous enzymes involved in microtubule detyrosination, whose mode of action is little known. Here, we show in reconstituted systems and cells that VASH1-SVBP and VASH2-SVBP drive the global and local detyrosination of microtubules, respectively. We solved the cryo-electron microscopy structure of VASH2-SVBP bound to microtubules, revealing a different microtubule-binding configuration of its central catalytic region compared to VASH1-SVBP. We show that the divergent mode of detyrosination between the two enzymes is correlated with the microtubule-binding properties of their disordered N- and C-terminal regions. Specifically, the N-terminal region is responsible for a significantly longer residence time of VASH2-SVBP on microtubules compared to VASH1-SVBP. We suggest that this VASH region is critical for microtubule detachment and diffusion of VASH-SVBP enzymes on lattices. Our results suggest a mechanism by which VASH1-SVBP and VASH2-SVBP could generate distinct microtubule subpopulations and confined areas of detyrosinated lattices to drive various microtubule-based cellular functions.<br /> (© 2022 Ramirez-Rios et al.)

Details

Language :
English
ISSN :
1540-8140
Volume :
222
Issue :
2
Database :
MEDLINE
Journal :
The Journal of cell biology
Publication Type :
Academic Journal
Accession number :
36512346
Full Text :
https://doi.org/10.1083/jcb.202205096